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Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes
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Genetics
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JoVE Journal Genetics
Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes

Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes

English

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1,060 Views

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05:34 min

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July 14, 2023

DOI:

10.3791/65472-v

DOI:
10.3791/65472-v

•

05:34 min
July 14, 2023

•

7 Views
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1Unità di Genetica, Dipartimento di Biologia,University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology,Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico

Summary

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This protocol presents a method to assess the formation and repair of DNA double-strand breaks through the simultaneous detection of γH2AX and 53BP1 foci in interphase nuclei of bleomycin-treated human peripheral lymphocytes.

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Dual ImmunofluorescenceγH2AX53BP1Human Peripheral LymphocytesDNA DamageDouble-strand BreaksBleomycinCell CultureFixationAntibody StainingFluorescence Microscopy

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Falaschi, A., Chiaramonte, A., Testi, S., Scarpato, R. Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes. J. Vis. Exp. (197), e65472, doi:10.3791/65472 (2023).

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