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Journal
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Genetics
/
A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages
/
High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes
JoVE Journal
Genetics
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JoVE Journal
Genetics
High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes
High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes
A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages
DOI:
10.3791/65264-v
•
00:50 min
•
August 04, 2023
•
Julia Craft
,
Tina Truong
,
Bennett H. Penn
2
1
Department of Internal Medicine
,
University of California, Davis
,
2
Department of Medical Microbiology and Immunology
,
University of California, Davis
Tags
Keyword Extraction:Gene Disruption
Primary Bone Marrow-derived Macrophages
Electroporated Cas9-sgRNA Complexes
CRISPR
Gene Editing
Macrophages
RNAi
Immunology
Gene Function
Sanger Sequencing
Ribonucleoprotein Complexes
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