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A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages
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Author Spotlight: Efficient CRISPR/Cas9 Genome Editing in Bone Marrow-Derived Macrophages for Precise Gene Disruption
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JoVE Journal 遺伝学
High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes

Author Spotlight: Efficient CRISPR/Cas9 Genome Editing in Bone Marrow-Derived Macrophages for Precise Gene Disruption

A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages

DOI:
10.3791/65264-v

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00:50 min

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August 04, 2023

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, ,
1内科学科,University of California, Davis, 2Department of Medical Microbiology and Immunology,University of California, Davis

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Keyword Extraction:Gene DisruptionPrimary Bone Marrow-derived MacrophagesElectroporated Cas9-sgRNA ComplexesCRISPRGene EditingMacrophagesRNAi免疫学Gene FunctionSanger SequencingRibonucleoprotein Complexes

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Author Spotlight: Efficient CRISPR/Cas9 Genome Editing in Bone Marrow-Derived Macrophages for Precise Gene Disruption

Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages

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