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A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages
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Perturbation génique à haute efficacité dans les macrophages primaires dérivés de la moelle osseuse à l’aide de complexes d’ARNsg Cas9-sgRNA électroporés
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Genetics
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JoVE Journal Genetics
High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes
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Perturbation génique à haute efficacité dans les macrophages primaires dérivés de la moelle osseuse à l’aide de complexes d’ARNsg Cas9-sgRNA électroporés

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854 Views

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00:50 min

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August 04, 2023

DOI:

10.3791/65264-v

DOI:
10.3791/65264-v

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00:50 min
August 04, 2023

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17 Views
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1Department of Internal Medicine,University of California, Davis, 2Department of Medical Microbiology and Immunology,University of California, Davis

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Keyword Extraction:Gene DisruptionPrimary Bone Marrow-derived MacrophagesElectroporated Cas9-sgRNA ComplexesCRISPRGene EditingMacrophagesRNAiImmunologyGene FunctionSanger SequencingRibonucleoprotein Complexes

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Craft, J., Truong, T., Penn, B. H. High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes. J. Vis. Exp. (198), e65264, doi:10.3791/65264 (2023).

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