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Isolamento de Vesículas Extracelulares de Baixo Conteúdo de Endotoxinas Derivadas de Linhagens Celulares Cancerígenas
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

Isolamento de Vesículas Extracelulares de Baixo Conteúdo de Endotoxinas Derivadas de Linhagens Celulares Cancerígenas

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824 Views

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06:28 min

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February 17, 2023

DOI:

10.3791/65062-v

DOI:
10.3791/65062-v

•

06:28 min
February 17, 2023

•

4 Views
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1Department of Clinical Immunology,Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences,Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Summary

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O protocolo proposto inclui diretrizes sobre como evitar a contaminação com endotoxina durante o isolamento de vesículas extracelulares de sobrenadantes de cultura celular e como avaliá-las adequadamente.

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Babula, A., Siemińska, I., Baj-Krzyworzeka, M. Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines. J. Vis. Exp. (192), e65062, doi:10.3791/65062 (2023).

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