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真菌β-葡聚糖的分离纯化作为胶质母细胞瘤的免疫治疗策略
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Neuroscience
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JoVE Journal Neuroscience
Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
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真菌β-葡聚糖的分离纯化作为胶质母细胞瘤的免疫治疗策略

DOI:
10.3791/64924-v

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10:02 min

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June 02, 2023

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, , , , , , , ,
1Department of Biochemistry and Molecular Biology, Faculty of Pharmacy,Universidad de Sevilla, 2Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío, CSIC,Universidad de Sevilla

Chapters

  • 00:04Introduction
  • 00:52Isolation of Fungal β-Glucans
  • 05:22In Vitro Study of β-Glucan-Induced Microglia Stimulation
  • 06:31Results: β-Glucan Purification and Their Potential Effects on Microglia Activation
  • 08:50Conclusion

Summary

Automatic Translation

Automatic Translation

本协议描述了四种不同真菌β-葡聚糖的纯化步骤和后续研究，作为增强小胶质细胞对胶质母细胞瘤细胞的抗肿瘤特性的潜在免疫调节分子。

Tags

Beta-glucanFungal PolysaccharidesImmunotherapyGlioblastomaMicrogliaMushroom ExtractsPleurotusHericiumGanodermaSoluble Mushroom PolysaccharidesInsoluble Mushroom PolysaccharidesAlpha-amylaseGlucoamylaseAlkalaseExtractionPurification

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