The present protocol describes the purification steps and subsequent studies of four different fungal β-glucans as potential immunomodulatory molecules that enhance the anti-tumoral properties of microglia against glioblastoma cells.
Folgado-Dorado, C., Caracena-De La Corte, J., Aguilera-Velázquez, J. R., Santana-Villalona, R., Rivera-Ramos, A., Carbonero-Aguilar, M. P., Talaverón, R., Bautista, J., Sarmiento Soto, M. Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma. J. Vis. Exp. (196), e64924, doi:10.3791/64924 (2023).