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Aislamiento y cultivo de somas vestibulares y ganglionares espirales de roedores neonatales para registros de patch-clamp
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Neuroscience
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JoVE Journal Neuroscience
Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings
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Aislamiento y cultivo de somas vestibulares y ganglionares espirales de roedores neonatales para registros de patch-clamp

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921 Views

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11:05 min

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April 21, 2023

DOI:

10.3791/64908-v

DOI:
10.3791/64908-v

•

11:05 min
April 21, 2023

•

9 Views
, , , , ,
1Caruso Department of Otolaryngology Head & Neck Surgery, Zilkha Neurogenetics Institute, Hearing and Communications Neuroscience Training Program,University of Southern California

Summary

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A continuación se presentan métodos que proporcionan instrucciones detalladas para la disección, la disociación, el cultivo y el registro de pinzas de parche de las neuronas ganglionares vestibulares y ganglionares espirales del oído interno.

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Iyer, M. R., Ventura, C., Bronson, D., Nowak, N., Regalado, K., Kalluri, R. Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings. J. Vis. Exp. (194), e64908, doi:10.3791/64908 (2023).

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