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Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings
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神经科学
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Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings

Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings

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11:05 min

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April 21, 2023

DOI:

10.3791/64908-v

DOI:
10.3791/64908-v

•

11:05 min
April 21, 2023

•

9 Views
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1Caruso Department of Otolaryngology Head & Neck Surgery, Zilkha Neurogenetics Institute, Hearing and Communications Neuroscience Training Program,University of Southern California

Summary

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Presented here are methods providing detailed instructions for dissecting, dissociating, culturing, and patch-clamp recording from vestibular ganglion and spiral ganglion neurons of the inner ear.

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Iyer, M. R., Ventura, C., Bronson, D., Nowak, N., Regalado, K., Kalluri, R. Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings. J. Vis. Exp. (194), e64908, doi:10.3791/64908 (2023).

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