Journal
/
Neurociencias
/
Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings

Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings

921 Views

•

11:05 min

•

April 21, 2023

DOI:

10.3791/64908-v

DOI:
10.3791/64908-v

•

11:05 min
April 21, 2023

•

9 Views
, , , , ,
1Caruso Department of Otolaryngology Head & Neck Surgery, Zilkha Neurogenetics Institute, Hearing and Communications Neuroscience Training Program,University of Southern California

Summary

Automatically generated

Presented here are methods providing detailed instructions for dissecting, dissociating, culturing, and patch-clamp recording from vestibular ganglion and spiral ganglion neurons of the inner ear.

Explore More Videos

VestibularSpiral GanglionPatch-clampNeonatal RodentsAuditory Afferent NeuronsCell BodiesIon ChannelsNeurotransmitter ReceptorsIn VitroVoltage-dependent PropertiesCell DiversityDissociationOtic CapsuleAuditory NerveSuperior GanglionInferior GanglionPeripheral Nerve BranchUtricle

Read Article

Cite this Article

Iyer, M. R., Ventura, C., Bronson, D., Nowak, N., Regalado, K., Kalluri, R. Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings. J. Vis. Exp. (194), e64908, doi:10.3791/64908 (2023).

Download .ris file

Copy

Share Video

.

Copy