Journal
/
Biology
/
Isolamento de núcleos de tecido hepático congelado por flash para multiômica unicelular
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Isolamento de núcleos de tecido hepático congelado por flash para multiômica unicelular

português

Automatically Generated

4,546 Views

•

09:09 min

•

December 09, 2022

DOI:

10.3791/64792-v

DOI:
10.3791/64792-v

•

09:09 min
December 09, 2022

•

75 Views
, , ,
1Helmholtz Pioneer Campus (HPC),Helmholtz Munich, 2Institute of Computational Biology, Computational Health Department,Helmholtz Munich, 3Division of Infectious Diseases and Tropical Medicine,Ludwig-Maximillian-Universität Klinikum, 4TUM School of Medicine,Technical University of Munich

Summary

Automatically generated

Aqui, apresentamos um protocolo para isolar núcleos de tecidos hepáticos arquivados congelados por flash para RNA-seq, ATAC-seq de núcleo único e multiômica articular (RNA-seq e ATAC-seq).

Explore More Videos

Nuclei IsolationSingle-cell MultiomicsDensity Gradient CentrifugationIodixanolCell StrainerBio-archived Human Liver SamplesMouse ModelNon-human Primate

Read Article

Cite this Article

Strzelecki, M., Yin, K., Talavera-López, C., Martinez-Jimenez, C. P. Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics. J. Vis. Exp. (190), e64792, doi:10.3791/64792 (2022).

Download .ris file

Copy

Share Video

.

Copy