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Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
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Biologie
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JoVE Journal Biologie
Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

4,554 Views

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09:09 min

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December 09, 2022

DOI:

10.3791/64792-v

DOI:
10.3791/64792-v

•

09:09 min
December 09, 2022

•

75 Views
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1Helmholtz Pioneer Campus (HPC),Helmholtz Munich, 2Institute of Computational Biology, Computational Health Department,Helmholtz Munich, 3Division of Infectious Diseases and Tropical Medicine,Ludwig-Maximillian-Universität Klinikum, 4TUM School of Medicine,Technical University of Munich

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Here, we present a protocol for isolating nuclei from flash-frozen, archived liver tissues for single-nucleus RNA-seq, ATAC-seq, and joint multiomics (RNA-seq and ATAC-seq).

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Strzelecki, M., Yin, K., Talavera-López, C., Martinez-Jimenez, C. P. Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics. J. Vis. Exp. (190), e64792, doi:10.3791/64792 (2022).

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