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Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

JoVE Revista
Biología

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JoVE Revista Biología

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

4,194 Views

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09:09 min

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December 09, 2022

DOI:

10.3791/64792-v

DOI:

10.3791/64792-v

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09:09 min

December 09, 2022

•

17 Views

Mateusz Strzelecki⁴, Kelvin Yin, Carlos Talavera-López³, Celia P. Martinez-Jimenez⁴

¹Helmholtz Pioneer Campus (HPC),Helmholtz Munich, ²Institute of Computational Biology, Computational Health Department,Helmholtz Munich, ³Division of Infectious Diseases and Tropical Medicine,Ludwig-Maximillian-Universität Klinikum, ⁴TUM School of Medicine,Technical University of Munich

Tags

Nuclei Isolation Single-cell Multiomics Density Gradient Centrifugation Iodixanol Cell Strainer Bio-archived Human Liver Samples Mouse Model Non-human Primate

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