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Agrobacterium tumefaciens-Dar Yapraklı Muzun Aracılı Genetik Transformasyonu
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Biology
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JoVE Journal Biology
Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain
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Agrobacterium tumefaciens-Dar Yapraklı Muzun Aracılı Genetik Transformasyonu

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3,096 Views

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07:50 min

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March 17, 2023

DOI:

10.3791/64777-v

DOI:
10.3791/64777-v

•

07:50 min
March 17, 2023

•

38 Views
, , , , , , ,
1Department of Agronomy, Center for Plant Biology,Purdue University, 2Department of Biology,Purdue University, 3Department of Horticulture, Faculty of Agricultural Sciences,University of the Punjab, 4Department of Botany and Plant Pathology, Center for Plant Biology,Purdue University

Summary

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Çeşitli çalışma alanlarında model bir tür olarak çok yönlü uygulaması nedeniyle, dar yapraklı muzda (Plantago lanceolata) genetik dönüşüm araç setine ihtiyaç vardır. Burada, Agrobacterium tumefaciens aracılı transformasyon kullanılarak,% 20'lik bir dönüşüm verimliliğine sahip stabil transgenik çizgilerle sonuçlanan bir protokol sunulmuştur.

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Cite this Article

Levengood, H., Dou, Y., Fan, J., Bajszar, A., Huang, J., Abbas, S. M., Zhou, Y., Zhang, C. Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain. J. Vis. Exp. (193), e64777, doi:10.3791/64777 (2023).

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