'Dergi'
/
Biyoloji
/
Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

DOI:
10.3791/64777-v

•

07:50 min

•

March 17, 2023

•

, , , , , , ,
1Department of Agronomy, Center for Plant Biology,Purdue University, 2'Biyoloji Bölümü',Purdue University, 3Department of Horticulture, Faculty of Agricultural Sciences,University of the Punjab, 4Department of Botany and Plant Pathology, Center for Plant Biology,Purdue University

'Bölümler'

  • 00:04Introduction
  • 00:32Plant Material Propagation for Transformation
  • 02:05Agrobacterium tumefaciens Preparation
  • 03:34Transformation of Plantago Roots
  • 04:55Selection and Whole Plant Regeneration
  • 05:38Soil Transfer
  • 06:09Results: Agrobacterium tumefaciens-Mediated Genetic Transformation
  • 07:25Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Because of its versatile application as a model species in various fields of study, there is a need for a genetic transformation toolkit in narrowleaf plantain (Plantago lanceolata). Here, using Agrobacterium tumefaciens-mediated transformation, a protocol is presented that results in stable transgenic lines with a transformation efficiency of 20%.

'Etiketler'

Agrobacterium TumefaciensGenetic TransformationNarrowleaf PlantainPlantago SpeciesPlant Vascular BiologyStress PhysiologyExplantsPlantago LanceolataEthanolSodium HypochloriteMS MediumPetri DishGerminationAgrobacteriumPlasmidLB PlateSelection AgentLiquid LB

Article

Embed

'ÇALMA LİSTESİNE EKLE'

Usage Statistics

'İlgili Videolar'

Method for Culture of Early Chick Embryos ex vivo (New Culture)

Double Whole Mount in situ Hybridization of Early Chick Embryos

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate

DNA Electroporation, Isolation and Imaging of Myofibers

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Author Spotlight: Streamlining Composite Plant Production via Agrobacterium rhizogenes-Mediated Hairy Root Transformation

Author Spotlight: Tissue Culture-Free Agrobacterium-Mediated Transformation in Large Woody Plants

Read Article