Journal
/
Biologie
/
Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

2,993 Views

•

07:50 min

•

March 17, 2023

DOI:

10.3791/64777-v

DOI:
10.3791/64777-v

•

07:50 min
March 17, 2023

•

38 Views
, , , , , , ,
1Department of Agronomy, Center for Plant Biology,Purdue University, 2Department of Biology,Purdue University, 3Department of Horticulture, Faculty of Agricultural Sciences,University of the Punjab, 4Department of Botany and Plant Pathology, Center for Plant Biology,Purdue University

Summary

Automatically generated

Because of its versatile application as a model species in various fields of study, there is a need for a genetic transformation toolkit in narrowleaf plantain (Plantago lanceolata). Here, using Agrobacterium tumefaciens-mediated transformation, a protocol is presented that results in stable transgenic lines with a transformation efficiency of 20%.

Explore More Videos

Agrobacterium TumefaciensGenetic TransformationNarrowleaf PlantainPlantago SpeciesPlant Vascular BiologyStress PhysiologyExplantsPlantago LanceolataEthanolSodium HypochloriteMS MediumPetri DishGerminationAgrobacteriumPlasmidLB PlateSelection AgentLiquid LB

Read Article

Cite this Article

Levengood, H., Dou, Y., Fan, J., Bajszar, A., Huang, J., Abbas, S. M., Zhou, Y., Zhang, C. Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain. J. Vis. Exp. (193), e64777, doi:10.3791/64777 (2023).

Download .ris file

Copy

Share Video

.

Copy