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In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells
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Neuroscience
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JoVE Journal Neuroscience
In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

DOI:
10.3791/64768-v

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08:57 min

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February 10, 2023

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, , , , , ,
1Department of Neurology,Ruhr-University Bochum, St. Josef Hospital

Chapters

  • 00:05Introduction
  • 00:49Sciatic Nerve Preparation and Refurbishment
  • 02:18Enzymatic Digestion of the Sciatic Nerve
  • 03:04Cell Separation
  • 03:59Schwann Cell Magnetic Separation
  • 04:56Dorsal Root Ganglia (DRG) Preparation
  • 06:04DRG Cell Culture and Coculture with Schwann Cells
  • 07:13Results: Myelination in Coculture of DRG Explants and Schwann Cells
  • 08:17Conclusion

Summary

Automatic Translation

Automatic Translation

In the coculture system of dorsal root ganglia and Schwann cells, myelination of the peripheral nervous system can be studied. This model provides experimental opportunities to observe and quantify peripheral myelination and to study the effects of compounds of interest on the myelin sheath.

Tags

Keywords: In Vitro MyelinationPeripheral AxonsDorsal Root Ganglion ExplantsSchwann CellsPeripheral Nervous SystemAxon-Schwann Cell InteractionsMyelination QuantificationDRG Explant CulturesNeurodegenerative DiseasesPeripheral Nerve SystemSciatic NerveNerve FibersEnzymatic Digestion

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