Journal
/
神经科学
/
In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

DOI:
10.3791/64768-v

•

08:57 min

•

February 10, 2023

•

, , , , , ,
1Department of Neurology,Ruhr-University Bochum, St. Josef Hospital

Chapters

  • 00:05Introduction
  • 00:49Sciatic Nerve Preparation and Refurbishment
  • 02:18Enzymatic Digestion of the Sciatic Nerve
  • 03:04Cell Separation
  • 03:59Schwann Cell Magnetic Separation
  • 04:56Dorsal Root Ganglia (DRG) Preparation
  • 06:04DRG Cell Culture and Coculture with Schwann Cells
  • 07:13Results: Myelination in Coculture of DRG Explants and Schwann Cells
  • 08:17Conclusion

Summary

自动翻译

自动翻译

In the coculture system of dorsal root ganglia and Schwann cells, myelination of the peripheral nervous system can be studied. This model provides experimental opportunities to observe and quantify peripheral myelination and to study the effects of compounds of interest on the myelin sheath.

Tags

Keywords: In Vitro MyelinationPeripheral AxonsDorsal Root Ganglion ExplantsSchwann CellsPeripheral Nervous SystemAxon-Schwann Cell InteractionsMyelination QuantificationDRG Explant CulturesNeurodegenerative DiseasesPeripheral Nerve SystemSciatic NerveNerve FibersEnzymatic Digestion

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/64768?article_type=v

Live Imaging of Dorsal Root Axons after Rhizotomy
check_url/cn/64768?article_type=v

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons
check_url/cn/64768?article_type=v

Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration
check_url/cn/64768?article_type=v

In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model
check_url/cn/64768?article_type=v

An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons
check_url/cn/64768?article_type=v

Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration
check_url/cn/64768?article_type=v

Laminotomy for Lumbar Dorsal Root Ganglion Access and Injection in Swine
check_url/cn/64768?article_type=v

Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry
check_url/cn/64768?article_type=v

Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion
check_url/cn/64768?article_type=v

Rapid Isolation of Dorsal Root Ganglion Macrophages

Read Article