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Stratégie basée sur CRISPR en une étape pour le marquage des gènes endogènes chez Drosophila melanogaster
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Neuroscience
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JoVE Journal Neuroscience
One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
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Stratégie basée sur CRISPR en une étape pour le marquage des gènes endogènes chez Drosophila melanogaster

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342 Views

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07:23 min

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January 26, 2024

DOI:

10.3791/64729-v

DOI:
10.3791/64729-v

•

07:23 min
January 26, 2024

•

19 Views
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1Department of Cell and Developmental Biology,University of Michigan, 2Cellular and Molecular Biology Program,University of Michigan, 3Michigan Neuroscience Institute Affiliate,University of Michigan

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CRISPRGene TaggingDrosophila MelanogasterHomologous RecombinationFluorescent ProteinKnock-inSgRNADonor VectorCloningPlasmidSanger Sequencing

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Xiao, Y., Yuan, Y., Yadlapalli, S. One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster. J. Vis. Exp. (203), e64729, doi:10.3791/64729 (2024).

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