Journal
/
Neurociencias
/
One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster

373 Views

•

07:23 min

•

January 26, 2024

DOI:

10.3791/64729-v

DOI:
10.3791/64729-v

•

07:23 min
January 26, 2024

•

5 Views
, ,
1Department of Cell and Developmental Biology,University of Michigan, 2Cellular and Molecular Biology Program,University of Michigan, 3Michigan Neuroscience Institute Affiliate,University of Michigan

Summary

Automatically generated

Here, we present a simplified endogenous gene tagging protocol for Drosophila, which utilizes a PCR-based technique for marker-free identification of successful genetic modifications, facilitating the development of stable knock-in lines.

Explore More Videos

CRISPRGene TaggingDrosophila MelanogasterHomologous RecombinationFluorescent ProteinKnock-inSgRNADonor VectorCloningPlasmidSanger Sequencing

Read Article

Cite this Article

Xiao, Y., Yuan, Y., Yadlapalli, S. One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster. J. Vis. Exp. (203), e64729, doi:10.3791/64729 (2024).

Download .ris file

Copy

Share Video

.

Copy