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One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
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One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
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07:23 min
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January 26, 2024
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Here, we present a simplified endogenous gene tagging protocol for Drosophila, which utilizes a PCR-based technique for marker-free identification of successful genetic modifications, facilitating the development of stable knock-in lines.
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Xiao, Y., Yuan, Y., Yadlapalli, S. One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster. J. Vis. Exp. (203), e64729, doi:10.3791/64729 (2024).
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