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Journal
/
Developmental Biology
/
表征胎儿小鼠结构性心脏缺陷的管道
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse
Please note that all translations are automatically generated.
Click here for the English version.
表征胎儿小鼠结构性心脏缺陷的管道
DOI:
10.3791/64582-v
•
08:19 min
•
December 16, 2022
•
Carla Guzman-Moreno*
1
,
Peizhao Zhang*
2
,
Olivia R. Phillips*
1
,
Mathias Block*
3
,
Benjamin J. Glennon*
1
,
Meghan Holbrook
,
Lauren Weigand
,
Cecilia W. Lo
,
Jiuann-Huey I. Lin
4
1
Department of Developmental Biology
,
University of Pittsburgh
,
2
Department of Biological Sciences
,
University of Pittsburgh
,
3
Département de Biologie
,
École Normale Supérieure de Lyon
,
4
Department of Critical Care Medicine
,
University of Pittsburgh
Chapters
00:05
Introduction
01:05
Fetal Echocardiogram
01:46
Necropsy
02:41
Episcopic Confocal Microscopy (ECM)
04:01
Three-Dimensional (3D) Reconstruction
06:14
Results: Analyzing Cardiac Anomalies Using Echo and ECM
07:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文详细介绍了使用胎儿超声心动图、尸检和使用会落共聚焦显微镜 (ECM) 的会射荧光图像捕获 (EFIC) 然后进行三维 (3D) 重建的小鼠先天性心脏病 (CHD) 诊断方法。
Tags
Fetal Mouse
Congenital Heart Defects
Echocardiography
Necropsy
Confocal Microscopy
Anatomical Changes
Structural Birth Defects
Ultrasound
Color Doppler
Sample Preparation
Laser Confocal Imaging
Image Acquisition
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