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Una pipeline per caratterizzare i difetti cardiaci strutturali nel topo fetale
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Developmental Biology
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JoVE Journal Developmental Biology
A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse
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Una pipeline per caratterizzare i difetti cardiaci strutturali nel topo fetale

italiano

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1,641 Views

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08:19 min

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December 16, 2022

DOI:

10.3791/64582-v

DOI:
10.3791/64582-v

•

08:19 min
December 16, 2022

•

1 Views
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1Department of Developmental Biology,University of Pittsburgh, 2Department of Biological Sciences,University of Pittsburgh, 3Département de Biologie,École Normale Supérieure de Lyon, 4Department of Critical Care Medicine,University of Pittsburgh

Summary

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Questo articolo descrive in dettaglio i metodi diagnostici di cardiopatia congenita murina (CHD) utilizzando l'ecocardiografia fetale, la necroscopia e l'acquisizione di immagini a fluorescenza episcopica (EFIC) utilizzando la microscopia confocale episcopica (ECM) seguita da ricostruzione tridimensionale (3D).

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Cite this Article

Guzman-Moreno, C., Zhang, P., Phillips, O. R., Block, M., Glennon, B. J., Holbrook, M., Weigand, L., Lo, C. W., Lin, J. I. A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse. J. Vis. Exp. (190), e64582, doi:10.3791/64582 (2022).

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