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Propagação de vírus e quantificação colorimétrica baseada em células
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Immunology and Infection
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JoVE Journal Immunology and Infection
Virus Propagation and Cell-Based Colorimetric Quantification

Propagação de vírus e quantificação colorimétrica baseada em células

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431 Views

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07:26 min

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April 07, 2023

DOI:

10.3791/64578-v

DOI:
10.3791/64578-v

•

07:26 min
April 07, 2023

•

6 Views
, , , ,
1Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE),Universiti Malaya, 2Department of Medical Microbiology, Faculty of Medicine,Universiti Malaya

Summary

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O presente protocolo descreve a propagação do vírus Zika (ZIKV) em células Vero African green monkey kidney e a quantificação do ZIKV usando métodos de imunodetecção colorimétrica baseados em células nos formatos de 24 e 96 poços (alto rendimento).

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Tan, J., Wong, J., Zainal, N., AbuBakar, S., Tan, K. Virus Propagation and Cell-Based Colorimetric Quantification. J. Vis. Exp. (194), e64578, doi:10.3791/64578 (2023).

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