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Virus Propagation and Cell-Based Colorimetric Quantification
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Virus Propagation and Cell-Based Colorimetric Quantification

Virus Propagation and Cell-Based Colorimetric Quantification

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07:26 min

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April 07, 2023

DOI:

10.3791/64578-v

DOI:
10.3791/64578-v

•

07:26 min
April 07, 2023

•

6 Views
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1Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE),Universiti Malaya, 2Department of Medical Microbiology, Faculty of Medicine,Universiti Malaya

Summary

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The present protocol describes the propagation of Zika virus (ZIKV) in Vero African green monkey kidney cells and the quantification of ZIKV using cell-based colorimetric immunodetection methods in 24-well and 96-well (high throughput) formats.

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Virus PropagationCell-based Colorimetric QuantificationZika VirusAntibody RecognitionVirus SerotypesHigh-throughput ApplicationsAutomated Imaging SystemVero CellsCell CultureVirus InoculumVirus QuantificationSerial Dilution

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Cite this Article

Tan, J., Wong, J., Zainal, N., AbuBakar, S., Tan, K. Virus Propagation and Cell-Based Colorimetric Quantification. J. Vis. Exp. (194), e64578, doi:10.3791/64578 (2023).

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