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Virus Propagation and Cell-Based Colorimetric Quantification
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JoVE Journal 免疫学と感染
Virus Propagation and Cell-Based Colorimetric Quantification

Virus Propagation and Cell-Based Colorimetric Quantification

DOI:
10.3791/64578-v

•

07:26 min

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April 07, 2023

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, , , ,
1Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE),Universiti Malaya, 2Department of Medical Microbiology, Faculty of Medicine,Universiti Malaya

章

  • 00:00Introduction
  • 00:43Virus Propagation
  • 02:15Virus Quantification
  • 04:31Staining
  • 05:29Results: Foci Forming Assay for Zika Virus P6-740 in 24 and 96-Well Plates
  • 06:55Conclusion

概要

自動翻訳

自動翻訳

The present protocol describes the propagation of Zika virus (ZIKV) in Vero African green monkey kidney cells and the quantification of ZIKV using cell-based colorimetric immunodetection methods in 24-well and 96-well (high throughput) formats.

タグ

Virus PropagationCell-based Colorimetric QuantificationZika VirusAntibody RecognitionVirus SerotypesHigh-throughput ApplicationsAutomated Imaging SystemVero CellsCell CultureVirus InoculumVirus QuantificationSerial Dilution

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