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Journal
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Immunology and Infection
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使用微泡从人外周血单核细胞样品中分离、活化和扩增基于浮选的 T 细胞
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles
Please note that all translations are automatically generated.
Click here for the English version.
使用微泡从人外周血单核细胞样品中分离、活化和扩增基于浮选的 T 细胞
DOI:
10.3791/64573-v
•
06:37 min
•
December 23, 2022
•
Tiffany Snow
,
Jon Roussey
,
Casey Wegner
,
Brandon McNaughton
1
Research and Development
,
Akadeum Life Sciences
,
2
Akadeum Life Sciences
Chapters
00:04
Introduction
00:29
Isolation of T Cells with Microbubbles Using Positive Selection
01:51
Co-Stimulation (Activation) of the Positively Selected T Cells
02:52
Expansion of the Co-Stimulated Cells in Cell Culture Medium
03:28
Expansion of the T Cells (With or Without Prior Transduction)
04:02
Harvesting the T Cells and Flow Cytometry
05:16
Results: Assessment of Viable and Successfully Transduced Cells
05:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
本研究的目的是证明基于浮选的分离在分离、激活和扩增原代人T细胞方面的可行性。
Tags
T Cell Isolation
T Cell Activation
T Cell Expansion
Microbubbles
Buoyancy-based Selection
PBMC
CD3 Antibody
CD28 Antibody
Cell Therapy
Cell Manufacturing
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