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Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles
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JoVE 杂志 免疫与感染
Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

DOI:
10.3791/64573-v

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06:37 min

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December 23, 2022

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, , ,
1Research and Development,Akadeum Life Sciences, 2Akadeum Life Sciences

Chapters

  • 00:04Introduction
  • 00:29Isolation of T Cells with Microbubbles Using Positive Selection
  • 01:51Co-Stimulation (Activation) of the Positively Selected T Cells
  • 02:52Expansion of the Co-Stimulated Cells in Cell Culture Medium
  • 03:28Expansion of the T Cells (With or Without Prior Transduction)
  • 04:02Harvesting the T Cells and Flow Cytometry
  • 05:16Results: Assessment of Viable and Successfully Transduced Cells
  • 05:58Conclusion

Summary

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The objective of this study is to demonstrate the feasibility of flotation-based separation to isolate, activate, and expand primary human T cells.

Tags

T Cell IsolationT Cell ActivationT Cell ExpansionMicrobubblesBuoyancy-based SelectionPBMCCD3 AntibodyCD28 AntibodyCell TherapyCell Manufacturing

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