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Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder
章
- 00:04Introduction
- 00:48Sample Collection and Preparation of Sample Holders
- 02:03Wounding of the Eyes
- 02:54Sample Placement on the Holder and Sample Imaging
- 04:18Results: High‐Resolution Live Cell Imaging of Enucleated Globes
- 05:14Conclusion
The present work describes a novel experimental protocol that utilizes a 3D printed holder to enable high-resolution live cell imaging of enucleated globes. Through this protocol, the cellular calcium signaling activity in wounded corneal epithelium from ex vivo globes can be observed in real time.
