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Un modelo triple de cultivo celular primario de la barrera hematoencefálica humana para estudiar el accidente cerebrovascular isquémico in vitro
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Biochemistry
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JoVE Journal Biochemistry
A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro
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Un modelo triple de cultivo celular primario de la barrera hematoencefálica humana para estudiar el accidente cerebrovascular isquémico in vitro

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3,432 Views

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11:06 min

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October 06, 2022

DOI:

10.3791/64469-v

DOI:
10.3791/64469-v

•

11:06 min
October 06, 2022

•

8 Views
, , , , ,
1Department of Biochemistry and Molecular Biology, Miller School of Medicine,University of Miami, 2Institute of Physiotherapy and Health Sciences,The Jerzy Kukuczka Academy of Physical Education

Summary

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Aquí, describimos el método para establecer un modelo de cultivo celular triple de la barrera hematoencefálica basado en células endoteliales microvasculares primarias del cerebro humano, astrocitos y pericitos. Este modelo multicelular es adecuado para estudios de disfunción de unidades neurovasculares durante el ictus isquémico in vitro o para el cribado de candidatos a fármacos.

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Cite this Article

Fattakhov, N., Torices, S., Becker, S., Teglas, T., Naranjo, O., Toborek, M. A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro. J. Vis. Exp. (188), e64469, doi:10.3791/64469 (2022).

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