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Ablação a Laser Multifóton de Centros Organizadores de Microtúbulos Citoplasmáticos em Oócitos de Camundongos
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Ablação a Laser Multifóton de Centros Organizadores de Microtúbulos Citoplasmáticos em Oócitos de Camundongos

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1,380 Views

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08:24 min

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November 11, 2022

DOI:

10.3791/64439-v

DOI:
10.3791/64439-v

•

08:24 min
November 11, 2022

•

5 Views
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1Animal Sciences Research Center,University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core,University of Missouri

Summary

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Apresenta-se um protocolo otimizado que permite a depleção de centros organizadores de microtúbulos citoplasmáticos em ovócitos de camundongos durante a metáfase I utilizando um laser de femtossegundos no infravermelho próximo.

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Cite this Article

Londoño-Vásquez, D., Jurkevich, A., Balboula, A. Z. Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes. J. Vis. Exp. (189), e64439, doi:10.3791/64439 (2022).

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