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マウス卵母細胞における細胞質微小管組織化中心の多光子レーザーアブレーション
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
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マウス卵母細胞における細胞質微小管組織化中心の多光子レーザーアブレーション

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1,380 Views

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08:24 min

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November 11, 2022

DOI:

10.3791/64439-v

DOI:
10.3791/64439-v

•

08:24 min
November 11, 2022

•

5 Views
, ,
1Animal Sciences Research Center,University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core,University of Missouri

Summary

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近赤外フェムト秒レーザーを用いた中期I期にマウス卵母細胞の細胞質微小管組織化中心の枯渇を可能にする最適化されたプロトコルを提示します。

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Cite this Article

Londoño-Vásquez, D., Jurkevich, A., Balboula, A. Z. Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes. J. Vis. Exp. (189), e64439, doi:10.3791/64439 (2022).

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