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In vitro Ensayos de escisión utilizando Caspasas de Drosophila recombinantes purificadas para cribado de sustrato
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening
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In vitro Ensayos de escisión utilizando Caspasas de Drosophila recombinantes purificadas para cribado de sustrato

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1,264 Views

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08:16 min

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October 06, 2022

DOI:

10.3791/64392-v

DOI:
10.3791/64392-v

•

08:16 min
October 06, 2022

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2 Views
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1Department of Molecular, Cell and Cancer Biology,UMass Chan Medical School

Summary

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Aquí presentamos un protocolo para expresar y purificar las caspasas recombinantes de Drosophila Dronc y Drice, y su uso en ensayos de escisión in vitro .

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In Vitro Cleavage AssayCaspasesDrosophilaDroncRecombinant ProteinsSubstrate ScreeningCell LysisNi-NTA AgarosePurificationEnzymatic Activity

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Yarikipati, P., Bergmann, A. In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening. J. Vis. Exp. (188), e64392, doi:10.3791/64392 (2022).

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