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JoVE Journal Bioengineering
In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening
 

In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening

Article DOI: 10.3791/64392-v • 08:16 min • October 6th, 2022
October 6th, 2022 •
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1Department of Molecular, Cell and Cancer Biology, UMass Chan Medical School

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Here we present a protocol to express and purify recombinant Drosophila caspases Dronc and Drice, and their use in in vitro cleavage assays.

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In Vitro Cleavage Assays Purified Recombinant Drosophila Caspases Substrate Screening Initiator Caspase Dronc Putative Substrates High Throughput Screening Caspases From Other Organisms Enzymatic Activity Frozen Tubes Bacterial Cell Lysis Buffer Protease Inhibitors Lysozyme Benzonase
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