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In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening
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In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening
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October 06, 2022
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Here we present a protocol to express and purify recombinant Drosophila caspases Dronc and Drice, and their use in in vitro cleavage assays.
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Yarikipati, P., Bergmann, A. In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening. J. Vis. Exp. (188), e64392, doi:10.3791/64392 (2022).
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