Journal
/
Bioengineering
/
In vitro Ensaios de Clivagem usando Caspases de Drosophila Recombinante Purificada para Triagem de Substrato
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening

In vitro Ensaios de Clivagem usando Caspases de Drosophila Recombinante Purificada para Triagem de Substrato

português

Automatically Generated

1,264 Views

•

08:16 min

•

October 06, 2022

DOI:

10.3791/64392-v

DOI:
10.3791/64392-v

•

08:16 min
October 06, 2022

•

2 Views
,
1Department of Molecular, Cell and Cancer Biology,UMass Chan Medical School

Summary

Automatically generated

Aqui apresentamos um protocolo para expressar e purificar Drosophila caspases recombinantes Dronc e Drice, e seu uso em ensaios de clivagem in vitro .

Explore More Videos

In Vitro Cleavage AssayCaspasesDrosophilaDroncRecombinant ProteinsSubstrate ScreeningCell LysisNi-NTA AgarosePurificationEnzymatic Activity

Read Article

Cite this Article

Yarikipati, P., Bergmann, A. In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening. J. Vis. Exp. (188), e64392, doi:10.3791/64392 (2022).

Download .ris file

Copy

Share Video

.

Copy