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체외 기질 스크리닝을 위해 정제된 재조합 초파리 카스파제를 사용한 절단 분석
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening
Please note that all translations are automatically generated. Click here for the English version.

체외 기질 스크리닝을 위해 정제된 재조합 초파리 카스파제를 사용한 절단 분석

English

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1,191 Views

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08:16 min

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October 06, 2022

DOI:

10.3791/64392-v

DOI:
10.3791/64392-v

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08:16 min
October 06, 2022

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4 Views
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1Department of Molecular, Cell and Cancer Biology,UMass Chan Medical School

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In Vitro Cleavage AssayCaspasesDrosophilaDroncRecombinant ProteinsSubstrate ScreeningCell LysisNi-NTA AgarosePurificationEnzymatic Activity

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Yarikipati, P., Bergmann, A. In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening. J. Vis. Exp. (188), e64392, doi:10.3791/64392 (2022).

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