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Hersenventrikelmicro-injecties van lipopolysaccharide in larvale zebravissen om neuro-inflammatie en neurotoxiciteit te beoordelen
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity
DOI:

07:31 min

August 23, 2022

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Chapters

  • 00:05Introduction
  • 00:39Preparing and Mounting Zebrafish for Microinjections
  • 02:22Injecting the Brain Ventricle
  • 03:08Imaging
  • 03:43Determination of Gene Expression Markers
  • 04:44Zebrafish Locomotive Behavioral Assay
  • 05:34Results: Lipopolysaccharide-Induced Neuroinflammation to Assess the Levels of Proinflammatory Mediators
  • 07:02Conclusion

Summary

Automatic Translation

Dit protocol demonstreert de micro-injectie van lipopolysaccharide in het ventriculaire gebied van de hersenen in een zebravislarvemodel om de resulterende neuro-inflammatoire respons en neurotoxiciteit te bestuderen.

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