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Hjerneventrikulære mikroinjektioner af lipopolysaccharid i larvezebrafisk for at vurdere neuroinflammation og neurotoksicitet
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JoVE Journal Neuroscience
Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Hjerneventrikulære mikroinjektioner af lipopolysaccharid i larvezebrafisk for at vurdere neuroinflammation og neurotoksicitet

DOI:
10.3791/64313-v

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07:31 min

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August 23, 2022

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,
1State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences,University of Macau, Avenida da Universidade, 2Department of Pharmaceutical Sciences, Faculty of Health Sciences,University of Macau, Avenida da Universidade

Chapters

  • 00:05Introduction
  • 00:39Preparing and Mounting Zebrafish for Microinjections
  • 02:22Injecting the Brain Ventricle
  • 03:08Imaging
  • 03:43Determination of Gene Expression Markers
  • 04:44Zebrafish Locomotive Behavioral Assay
  • 05:34Results: Lipopolysaccharide-Induced Neuroinflammation to Assess the Levels of Proinflammatory Mediators
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol demonstrerer mikroinjektion af lipopolysaccharid i hjernens ventrikulære region i en zebrafisk larvemodel for at studere det resulterende neuroinflammatoriske respons og neurotoksicitet.

Tags

Keywords: Brain VentricleMicroinjectionLipopolysaccharideNeuroinflammationNeurotoxicityZebrafishIn Vivo ModelAnti-neuroinflammatory DrugsGlass CapillaryMicromanipulatorAgaroseAnesthesia

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