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Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
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Immunology and Infection
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JoVE Journal Immunology and Infection
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

English

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2,793 Views

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09:48 min

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July 27, 2022

DOI:

10.3791/64215-v

DOI:
10.3791/64215-v

•

09:48 min
July 27, 2022

•

11 Views
, , , , , ,
1Division of Pediatric Surgery,Oklahoma Children’s Hospital, 2Oklahoma University Health Sciences Center, 3Department of Surgery,University of Oklahoma Health Sciences Center

Summary

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The present protocol outlines a method that utilizes lucifer yellow in an apical-out enteroid model to determine intestinal permeability. This method can be used to determine paracellular permeability in enteroids that model inflammatory bowel diseases such as necrotizing enterocolitis.

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Cite this Article

Liebe, H., Schlegel, C., Cai, X., Golubkova, A., Leiva, T., Berry, W. L., Hunter, C. J. Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model. J. Vis. Exp. (185), e64215, doi:10.3791/64215 (2022).

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