Journal
/
Imunologia e Infecção
/
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

2,793 Views

•

09:48 min

•

July 27, 2022

DOI:

10.3791/64215-v

DOI:
10.3791/64215-v

•

09:48 min
July 27, 2022

•

11 Views
, , , , , ,
1Division of Pediatric Surgery,Oklahoma Children’s Hospital, 2Oklahoma University Health Sciences Center, 3Department of Surgery,University of Oklahoma Health Sciences Center

Summary

Automatically generated

The present protocol outlines a method that utilizes lucifer yellow in an apical-out enteroid model to determine intestinal permeability. This method can be used to determine paracellular permeability in enteroids that model inflammatory bowel diseases such as necrotizing enterocolitis.

Explore More Videos

Lucifer YellowApical-out Enteroid ModelIntestinal PermeabilityParacellular PathwaysNecrotizing EnterocolitisCell Recovery SolutionCentrifugation3D Model24-well PlateAO Enteroids

Read Article

Cite this Article

Liebe, H., Schlegel, C., Cai, X., Golubkova, A., Leiva, T., Berry, W. L., Hunter, C. J. Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model. J. Vis. Exp. (185), e64215, doi:10.3791/64215 (2022).

Download .ris file

Copy

Share Video

.

Copy