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Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
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Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
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09:48 min
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July 27, 2022
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The present protocol outlines a method that utilizes lucifer yellow in an apical-out enteroid model to determine intestinal permeability. This method can be used to determine paracellular permeability in enteroids that model inflammatory bowel diseases such as necrotizing enterocolitis.
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Liebe, H., Schlegel, C., Cai, X., Golubkova, A., Leiva, T., Berry, W. L., Hunter, C. J. Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model. J. Vis. Exp. (185), e64215, doi:10.3791/64215 (2022).
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