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La tecnica peel-blot: un metodo di preparazione dei campioni crio-EM per separare singoli strati da campioni biologici multistrato o concentrati
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Biochemistry
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JoVE Journal Biochemistry
The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples
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La tecnica peel-blot: un metodo di preparazione dei campioni crio-EM per separare singoli strati da campioni biologici multistrato o concentrati

italiano

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2,163 Views

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07:27 min

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June 29, 2022

DOI:

10.3791/64099-v

DOI:
10.3791/64099-v

•

07:27 min
June 29, 2022

•

2 Views
, ,
1School of Biological Sciences,Georgia Institute of Technology, 2Department of Structural Biology,Genentech, 3School of Chemistry and Biochemistry,Georgia Institute of Technology

Summary

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La tecnica peel-blot è un metodo di preparazione della griglia crio-EM che consente la separazione di campioni biologici multistrato e concentrati in singoli strati per ridurre lo spessore, aumentare la concentrazione del campione e facilitare l'elaborazione delle immagini.

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Cite this Article

Johnson, M. C., Grant, A. J., Schmidt-Krey, I. The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples. J. Vis. Exp. (184), e64099, doi:10.3791/64099 (2022).

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