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The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples
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The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples

The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples

2,162 Views

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07:27 min

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June 29, 2022

DOI:

10.3791/64099-v

DOI:
10.3791/64099-v

•

07:27 min
June 29, 2022

•

2 Views
, ,
1School of Biological Sciences,Georgia Institute of Technology, 2Department of Structural Biology,Genentech, 3School of Chemistry and Biochemistry,Georgia Institute of Technology

Summary

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The peel-blot technique is a cryo-EM grid preparation method that allows for the separation of multilayered and concentrated biological samples into single layers to reduce thickness, increase sample concentration, and facilitate image processing.

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Peel-Blot TechniqueCryo-EMSample PreparationMulti-Layered SamplesConcentrated SamplesSingle LayersThickness ReductionConcentration IncreaseImage ProcessingFilter PaperParaffin FilmSubmicron Filter PaperAnti-Capillary Forceps600 Mesh GridTrehalose SolutionMicaCarbon FilmGrid Orientation

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Johnson, M. C., Grant, A. J., Schmidt-Krey, I. The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples. J. Vis. Exp. (184), e64099, doi:10.3791/64099 (2022).

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