Journal
/
Biochimie
/
The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples

The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples

2,162 Views

•

07:27 min

•

June 29, 2022

DOI:

10.3791/64099-v

DOI:
10.3791/64099-v

•

07:27 min
June 29, 2022

•

2 Views
, ,
1School of Biological Sciences,Georgia Institute of Technology, 2Department of Structural Biology,Genentech, 3School of Chemistry and Biochemistry,Georgia Institute of Technology

Summary

Automatically generated

The peel-blot technique is a cryo-EM grid preparation method that allows for the separation of multilayered and concentrated biological samples into single layers to reduce thickness, increase sample concentration, and facilitate image processing.

Explore More Videos

Peel-Blot TechniqueCryo-EMSample PreparationMulti-Layered SamplesConcentrated SamplesSingle LayersThickness ReductionConcentration IncreaseImage ProcessingFilter PaperParaffin FilmSubmicron Filter PaperAnti-Capillary Forceps600 Mesh GridTrehalose SolutionMicaCarbon FilmGrid Orientation

Read Article

Cite this Article

Johnson, M. C., Grant, A. J., Schmidt-Krey, I. The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples. J. Vis. Exp. (184), e64099, doi:10.3791/64099 (2022).

Download .ris file

Copy

Share Video

.

Copy