Journal
/
Biochemistry
/
Reconstitutie van septineassemblage bij membranen om biofysische eigenschappen en functies te bestuderen
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
Please note that all translations are automatically generated. Click here for the English version.

Reconstitutie van septineassemblage bij membranen om biofysische eigenschappen en functies te bestuderen

Nederlands

Automatically Generated

2,088 Views

•

06:32 min

•

July 28, 2022

DOI:

10.3791/64090-v

DOI:
10.3791/64090-v

•

06:32 min
July 28, 2022

•

2 Views
, , ,
1Department of Biochemistry and Biophysics,University of North Carolina at Chapel Hill, 2Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, 3Department of Biology,University of North Carolina at Chapel Hill

Summary

Automatically generated

Celvrije reconstitutie is een belangrijk hulpmiddel geweest om de cytoskeletassemblage te begrijpen, en het werk in het afgelopen decennium heeft benaderingen vastgesteld om septinedynamica in minimale systemen te bestuderen. Hier worden drie complementaire methoden gepresenteerd om septineassemblage in verschillende membraancontexten te observeren: vlakke bilayers, sferische steunen en staafsteunen.

Explore More Videos

Membrane BindingMembrane ShapeProtein AssemblyLipid BilayerLiposomesPlasma CleaningUV-activated AdhesiveSupported Lipid BilayerSeptinsTIRF MicroscopySilica MicrospheresSUVs

Read Article

Cite this Article

Curtis, B. N., Vogt, E. J. D., Cannon, K. S., Gladfelter, A. S. Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions. J. Vis. Exp. (185), e64090, doi:10.3791/64090 (2022).

Download .ris file

Copy

Share Video

.

Copy