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在膜上重构隔膜组件以研究生物物理性质和功能
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Biochemistry
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JoVE Journal Biochemistry
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
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在膜上重构隔膜组件以研究生物物理性质和功能

English

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2,085 Views

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06:32 min

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July 28, 2022

DOI:

10.3791/64090-v

DOI:
10.3791/64090-v

•

06:32 min
July 28, 2022

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2 Views
, , ,
1Department of Biochemistry and Biophysics,University of North Carolina at Chapel Hill, 2Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, 3Department of Biology,University of North Carolina at Chapel Hill

Summary

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无细胞重建一直是了解细胞骨架组装的关键工具，并且在过去十年中的工作已经建立了研究最小系统中隔膜蛋白动力学的方法。这里介绍的是三种在不同膜环境中观察隔膜组装的互补方法：平面双层，球形支撑和棒状支撑。

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Cite this Article

Curtis, B. N., Vogt, E. J. D., Cannon, K. S., Gladfelter, A. S. Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions. J. Vis. Exp. (185), e64090, doi:10.3791/64090 (2022).

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