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Visualizando a dinâmica de acoplamento de actina e microtúbula in vitro por microscopia de fluorescência interna total (TIRF)
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy

Visualizando a dinâmica de acoplamento de actina e microtúbula in vitro por microscopia de fluorescência interna total (TIRF)

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2,820 Views

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08:44 min

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July 20, 2022

DOI:

10.3791/64074-v

DOI:
10.3791/64074-v

•

08:44 min
July 20, 2022

•

7 Views
1Department of Biochemistry & Molecular Biology, and Department of Neuroscience & Physiology,State University of New York (SUNY) Upstate Medical University

Summary

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Este protocolo é um guia para visualizar actina dinâmica e microtúbulos usando um ensaio de microscopia de fluorescência interna total in vitro (TIRF).

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Henty-Ridilla, J. L. Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy. J. Vis. Exp. (185), e64074, doi:10.3791/64074 (2022).

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