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Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy
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Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy

DOI:
10.3791/64074-v

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08:44 min

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July 20, 2022

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1Department of Biochemistry & Molecular Biology, and Department of Neuroscience & Physiology,State University of New York (SUNY) Upstate Medical University

Bölümler

  • 00:04Introduction
  • 00:40Coating Cleaned Coverslips with mPEG- and Biotin-PEG-Silane
  • 01:21Assembling Imaging Flow Chambers
  • 02:50Conditioning of Perfusion Chambers
  • 03:47Microscope Preparation
  • 04:40Preparation of Protein Reaction Mixes
  • 06:02Image Actin and Microtubule Dynamics
  • 06:43Results: Example Measurements of Microtubules and Actin Filament Dynamics
  • 08:14Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This protocol is a guide for visualizing dynamic actin and microtubules using an in vitro total internal fluorescence (TIRF) microscopy assay.

Etiketler

ActinMicrotubuleTIRF MicroscopyPEG-SilaneBiotin-PEG-SilaneCover SlipImaging ChamberEpoxy ResinPerfusion ChamberConditioning SolutionsTotal Internal Reflection Fluorescence

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