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Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy
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Biochemistry
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JoVE Journal Biochemistry
Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy

English

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2,819 Views

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08:44 min

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July 20, 2022

DOI:

10.3791/64074-v

DOI:
10.3791/64074-v

•

08:44 min
July 20, 2022

•

7 Views
1Department of Biochemistry & Molecular Biology, and Department of Neuroscience & Physiology,State University of New York (SUNY) Upstate Medical University

Summary

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This protocol is a guide for visualizing dynamic actin and microtubules using an in vitro total internal fluorescence (TIRF) microscopy assay.

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Henty-Ridilla, J. L. Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy. J. Vis. Exp. (185), e64074, doi:10.3791/64074 (2022).

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