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Neuroscience
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毫秒氢/氘交换质谱法用于生理条件下α-突触核蛋白结构动力学的研究
JoVE Journal
Neuroscience
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Neuroscience
Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions
Please note that all translations are automatically generated.
Click here for the English version.
毫秒氢/氘交换质谱法用于生理条件下α-突触核蛋白结构动力学的研究
DOI:
10.3791/64050-v
•
08:40 min
•
June 23, 2022
•
Neeleema Seetaloo
,
Jonathan J. Phillips
1
Living Systems Institute, Department of Biosciences
,
University of Exeter
Chapters
00:04
Introduction
00:41
Peptide Mapping Procedure
02:38
Millisecond Hydrogen/Deuterium Exchange Study
05:34
Data Processing and Data Analysis
07:19
Results: Measuring the Conformational Dynamics of aSyn
08:14
Conclusion
Summary
Automatic Translation
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Automatic Translation
单体α-突触核蛋白的结构集合影响其生理功能和理化性质。本方案描述了如何进行毫秒氢/氘交换质谱和随后的数据分析,以确定在生理条件下这种内在无序蛋白质的单体的构象信息。
Tags
Alpha-synuclein
Hydrogen/deuterium Exchange
Mass Spectrometry
Intrinsically Disordered Proteins
Peptide Mapping
Sample Preparation
Autosampler
HDX Robotics
Quench Buffer
Peptide Coverage Map
Fast HDX Prototype Instrument
HDX Data Analysis Software
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